Flow cytometry (FACS) staining protocol (Cell surface staining)
We recommend staining with ice cold reagents/solutions and at 4°C, since low temperature and presence of sodium azide prevent the modulation and internalization of surface antigens which can produce a loss of fluorescence intensity. Add 100 µl of cell suspension to each tube.
BestProtocols: Staining Cell Surface Targets for Flow Cytometry
Stains samples with a viability dye according to LIVE/DEAD staining protocol or BestProtocols: Viability Staining Protocol for Flow Cytometry. Note: Use a LIVE/DEAD Fixable Dead Cell Stain with NovaFluor dyes.
Cell Surface Immunofluorescence Staining Protocol Harvest Tissue or Cells: 1. Obtain desired tissue ( e.g . spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension in Cell Staining Buffer (BioLegend Cat. #420201). If using resuspend previously activated cultures in Cell Staining Buffer and proceed to Step 2.
Protocol: Cell Surface Antibody Staining for Flow Cytometry
There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic protocol for extracellular staining of cell surface epitopes in suspension cells for flow cytometry.
Surface and Intracellular Staining Protocols for Flow Cytometry …
Nov 9, 2018 · Here, we will discuss the various staining buffers that are used in flow cytometry. In addition, we have outlined detailed protocols for carrying out both surface and intracellular staining in human and rodent tissues such as whole blood and PBMCs.
Flow Cytometry Protocol for Cell Surface Markers - R&D Systems
Master flow cytometry staining of membrane-associated proteins on suspended cells with this protocol. Identify populations and perform FACS effectively!
BestProtocols: Staining Intracellular Antigens for Flow Cytometry
A modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular antigens at the single-cell level by flow cytometry.
flow cytometry and flow sorting results and can result in doublet events. This can be the result of more adherent cells, such as macrophages or adherent.
Flow Cytometry Protocols | Thermo Fisher Scientific - US
Get flow cytometry protocols for cell preparation, red blood cell lysis, staining cells, compensation beads, viability and cell proliferation.
Planning For Surface Staining Of Cells In Flow Cytometry
One of the most common assays in flow cytometry is the surface labeling of cells with antibodies. Often termed “immunophenotyping”, it allows the researcher to identify, count, and isolate cells of interest in a mix of input cells.