CRISPR-Cas based virus detection: Recent advances and …
WEBDec 12, 2021 · CRISPR-Cas systems, especially CRISPR-Cas12a and CRISPR-Cas13a, characterized by their sensitivity, specificity, high base resolution and programmability upon nucleic acid recognition, have been repurposed for molecular diagnostics, surging a new path forward in biosensing.
Latest Advances of Virology Research Using CRISPR/Cas9 …
WEBApr 28, 2021 · Using the CRISPR/Cas9-based gene editing approach, a highly efficient recombinant virus, containing CDV virus-like particles (VLPs) and concurrently expressing the matrix (M), H, and F genes, has been recently constructed, which can assemble CDV-VLPs and provide faster seroconversion and higher rates of …
CRISPR-Cas based virus detection: Recent advances and perspectives
WEBDec 1, 2021 · Rapid, sensitive, specific and field-deployable identification of causal viruses is critical for disease surveillance, control and treatment. The shortcomings of current methods create an impending need for developing novel biosensing platforms.
Diverse virus-encoded CRISPR-Cas systems include streamlined …
WEBCRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems.
CRISPR–Cas12-based detection of SARS-CoV-2 - Nature
WEBApr 16, 2020 · We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts.
The Use of CRISPR/Cas9 as a Tool to Study Human Infectious …
WEBAug 27, 2021 · To facilitate virus removals, the CRISPR/Cas9 system has already been customized to confer new antiviral capabilities into host animals either by modifying host genome or by directly targeting viral inherent factors in the form of DNA.
RNA-targeting CRISPR–Cas systems - Nature Reviews Microbiology
WEBSep 28, 2022 · CRISPR–Cas is a widespread adaptive immune system in bacteria and archaea that protects against viral infection by targeting specific invading nucleic acid sequences.
CRISPR/Cas9 therapeutics: progress and prospects
WEBJan 16, 2023 · CRISPR/ Cas9 is a highly effective gene-editing tool that is widely used in the scientific community. 6 The CRISPR/Cas9 system evolved naturally in bacteria and archaea as a defense mechanism...
The Application of CRISPR/Cas Systems for Antiviral Therapy
WEBOct 12, 2021 · In nature, CRISPR/Cas systems are the adaptive immune systems within bacteria that protect against invading viruses and foreign nucleic acids (Horvath and Barrangou, 2010). Therefore, the logic follows that it might not be a step too far to exploit them to protect humans against viral infections.
CRISPR-Cas-based techniques for pathogen detection: …
WEBAug 1, 2023 · CRISPR-Cas-based biosensing technology has the potential to be an ideal pathogen diagnostic tool. •. Technology's transition from the laboratory to the clinical diagnosis depends on standardization. Abstract. Background. Early detection of pathogen-associated diseases are critical for effective treatment.
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