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    SymbolGFP
    PfamPF01353
    Wikipedia
    https://en.wikipedia.org/wiki/Green_fluorescent_protein

    Green fluorescent protein - Wikipedia

    • The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. However, GFPs have been found in other organisms including corals, sea anemones… See more

    Background

    In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of luciferin, releasing light), was first purified from the jellyfish Aequorea victoria and its properties … See more

    In nature

    The purpose of both the (primary) bioluminescence (from aequorin's action on luciferin) and the (secondary) fluorescence of GFP in jellyfish is unknown. GFP is co-expressed with aequorin in small granules around the … See more

    Other fluorescent proteins

    There are many GFP-like proteins that, despite being in the same protein family as GFP, are not directly derived from Aequorea victoria. These include dsRed, eqFP611, Dronpa, TagRFPs, KFP, EosFP/Iris… See more

    Structure

    GFP has a beta barrel structure consisting of eleven β-strands with a pleated sheet arrangement, with an alpha helix containing the covalently bonded chromophore 4-(p-hydroxybenzylidene)imidazolidin-5-one (HBI) runni… See more

    Applications

    Green fluorescent protein may be used as a reporter gene.
    For example, GFP can be used as a reporter for environmental toxicity levels. This protein has been shown to be an effective w… See more

    Further reading

    • Pieribone V, Gruber D (2006). Aglow in the Dark: The Revolutionary Science of Biofluorescence. Cambridge: Belknap Press. ISBN 978-0-674-01921-8. OCLC 60321612. Popular science book describing … See more

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    Quick Facts
    Background
    In nature
    Other fluorescent proteins
    Structure
    Applications
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  1. Green fluorescent protein
    • According to 4 sources
    The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

    Green fluorescent protein …

    en.wikipedia.org
    The very aptly named green fluorescent protein — or GFP as it is almost universally known — is a barrel-shaped protein made up of 238 amino acids. Threaded through the long axis of the β-sheet barrel is an α-helix that contains a chromophore that is responsible for the emission of green light when GFP is exposed to either blue or ultaviolet light.

    Green fluorescent protein …

    nature.com
    The green fluorescent protein (GFP) is a monomeric cytoplasmic fluorescent protein that emits green fluorescent light upon exposure to the blue to ultraviolet (UV) spectral regions.

    Green Fluorescent Protein …

    sciencedirect.com
    Green fluorescent protein (GFP) is a bioluminescent polypeptide consisting of 238 residues isolated from the body of Aequorea victoria jellyfish. GFP converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light.

    Green Fluorescent Protein …

    proteopedia.org
  2. People also ask
    What does GFP stand for?
    The green fluorescent protein (GFP) is a monomeric cytoplasmic fluorescent protein that emits green fluorescent light upon exposure to the blue to ultraviolet (UV) spectral regions. You might find these chapters and articles relevant to this topic. T. Ould-Ely, ... D.E. Morse, in Comprehensive Biomaterials, 2011

    Green Fluorescent Protein - an overview | ScienceDirect Topics

    sciencedirect.com
    What is green fluorescent protein (GFP)?
    The very aptly named green fluorescent protein — or GFP as it is almost universally known — is a barrel-shaped protein made up of 238 amino acids. Threaded through the long axis of the β-sheet barrel is an α-helix that contains a chromophore that is responsible for the emission of green light when GFP is exposed to either blue or ultaviolet light.

    Green fluorescent protein - Nature Chemistry

    nature.com
    How many amino acids are in a GFP protein?
    Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long. The natural protein has 238 amino acids. Its molecular mass is 27 kD. [ 7 ]

    Green fluorescent protein - Wikipedia

    en.wikipedia.org
    Where is GFP expressed?
    GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells. Scientists Roger Y. Tsien, Osamu Shimomura, and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.

    Green fluorescent protein - Wikipedia

    en.wikipedia.org
    What is the molecular mass of a GFP gene?
    Its molecular mass is 27 kD. [ 7 ] Therefore, fusing the GFP gene to the gene of a protein of interest can significantly increase the protein's size and molecular mass, and can impair the protein's natural function or change its location or trajectory of transport within the cell. [ 8 ]

    Green fluorescent protein - Wikipedia

    en.wikipedia.org
    Why is GFP important?
    GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.

    The green fluorescent protein - PubMed

    pubmed.ncbi.nlm.nih.gov
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  3. Nature
    https://www.nature.com/articles/nchem.75

    Green fluorescent protein - Nature Chemistry

  4. Addgene Blog
    https://blog.addgene.org/plasmids-101-g…

    Fluorescent Proteins 101: Green Fluorescent Protein …

    Learn about GFP, a protein derived from jellyfish that emits green fluorescence and is widely used in molecular biology. Discover how GFP is engineered, applied, and visualized in various research fields.

  5. NobelPrize.org
    https://www.nobelprize.org/uploads/2013/06/...
    [PDF]

    The green fluorescent protein: discovery, expression and …

  6. ScienceDirect
    https://www.sciencedirect.com/topics/neuroscience/...

    Green Fluorescent Protein - an overview | ScienceDirect Topics

  8. PubMed
    https://pubmed.ncbi.nlm.nih.gov/9759496

    The green fluorescent protein - PubMed

  9. Jackson ImmunoResearch
    https://www.jacksonimmuno.com/.../trending-topics/introduction-to-gfp

    An Introduction to Green Fluorescent Protein (GFP)

  10. ScienceDirect
    https://www.sciencedirect.com/.../green-fluorescent-protein

    Green Fluorescent Protein - an overview | ScienceDirect Topics

  11. Nature
    https://www.nature.com/articles/s41587-022-01278-2

    A highly photostable and bright green fluorescent protein

  12. PubMed
    https://pubmed.ncbi.nlm.nih.gov/19771329

    GFP: from jellyfish to the Nobel prize and beyond - PubMed