Cloning vector - Wikipedia
WEBA cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. [1] . The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.
Cloning Vectors- Definition, Characteristics, Types, Uses
WEBSep 12, 2021 · In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed. Vectors can replicate autonomously and typically include features to facilitate the manipulation of DNA as well as a genetic marker for their selective recognition.
The art of vector engineering: towards the construction of ...
WEBSep 26, 2018 · For simplicity, vectors can be divided into three typical classes most commonly used by researchers: cloning vectors, expression vectors and reporter vectors (Fig. 2 B). Cloning vectors are the ones used to make numerous copies of a DNA of interest, keeping them stable inside a host organism (Rodriquez and Denhardt, 1988 ; …
8.5: Cloning DNA - Plasmid Vectors - Biology LibreTexts
WEBJun 20, 2023 · Molecular biologists use plasmids as vectors to contain, amplify, transfer, and sometimes express genes of interest that are present in the cloned DNA.
9.3: Cloning and Recombinant Expression - Biology LibreTexts
WEBJul 13, 2023 · All commonly used cloning vectors in molecular biology have key features necessary for their function, such as a suitable cloning site with restriction enzymes and a selectable marker. Others may have additional features specific to their use.
Vector (molecular biology) - Wikipedia
WEBIn molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence – usually DNA – into another cell, where it can be replicated and/or expressed. [1] A vector containing foreign DNA is termed recombinant DNA.
Isolating, Cloning, and Sequencing DNA - Molecular Biology of ...
WEBA virus or plasmid used in this way is known as a cloning vector, and the DNA propagated by insertion into it is said to have been cloned. To isolate a specific gene, one often begins by constructing a DNA library —a comprehensive collection of cloned DNA fragments from a cell, tissue, or organism.
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