Chromosomal and Nuclear Alterations in Root Tip Cells of Allium Cepa …
The growing root tips of the onion, Allium cepa provide a readily available source of material for studying the damaging effects of chemicals on chromosomes. This plant is useful for evaluating DNA damages that express such as CAs, disturbances in the mitotic cycle, nuclear alterations (NAs) and presence of MNi in meristem cells.
Among the endpoints of Allium cepa root chromosomal aberrations, detection of chromosomalaberration have been the most used one to detect genotoxicity/antigenotoxicity along the years.
the squeezed extracts of garri on root growth of Allium cepa. Maximum root growth was achieved in the control and without any morphological deformities. The roots were whitish in colour, straight and unbroken. At tested concentrations, root growth was highest at the 1% concentration of the 4-day fermented extract. On the other hand, mean root
Allium Cepa Root Chromosomal Aberration Assay: A Review
Mar 31, 2013 · The Allium cepa root chromosomal aberration assay is widely used to determine genotoxic and antigenotoxic effects of different plant extracts.
Allium cepa assay based comparative study of selected …
The extracts of harvested plants were used to grow the root of A. cepa following its standard method. The root tips were fixed, stained and examined under compound microscope (almost 300–400 dividing cells) to check the extent of chromosomal variations during various stages of …
The onion plant (Allium cepa L.) is a suitable indicator plant for the determination of potential genotoxic agents in the samples taken from the environment. The genotoxic level of the agent under study is reflected by structural changes of the chromosomes and their changed numbers.
The present study deals with the evaluation of antimitotic activity of leaf extracts of Azadirachta indica A. Juss. and Simarouba glauca DC. using Allium cepa root tip cells. 50% and 25% of leaf extracts of both the plants were prepared and onion root …
meristem into a cortex begins higher up in the root tip than the level at which differentiation begins in the plerome, so that periblem cells are the best source of meristematic division.
Allium Cepa Root Chromosomal Aberration Assay: A Review
Of all plant bioassays available, Allium cepa root chromosomal aberration assay is an efficient, most-easy and inexpensive tool that can be used for (i) assessment of genotoxicity of environmental mutagens and (ii) evaluation of anti-genotoxic potential of plant extracts.
All concentrations induced a number of chromosomal aberrations in the root tip cells. The mitotic index decreased significantly (p < 0.05) with increasing concentration. The cytotoxic effects showed strong concentration-dependent root growth …