Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Flow cytometry workflows. ... cell viability, and cell surface or intracellular protein expression. ... refined intra- and extracellular staining protocols, and best-in-class antibodies.

There are different staining techniques for flow cytometry. Among these is immunofluorescence staining, which can be direct or indirect. Direct immunofluorescence staining involves treating cells ...

While flow cytometry can reliably assess surface and intracellular marker expression ... and a CD4 receptor detection antibody were used along with intracellular staining to determine FOXO1 ...

Over the past 12 years, Mathi has implemented advanced flow cytometry techniques, including immunophenotyping (staining for cell surface markers), intracellular staining (ICS), and phospho-flow ...

Please use one of the following formats to cite this article in your essay, paper or report: APA. P, Surat. (2019, July 31). Detecting Cytokines using Intracellular Flow Cytometry.