The tumor suppressor p53 is a transcription factor that controls the expression of hundreds of genes. Emerging evidence suggests that the p53-induced RNA-binding protein ZMAT3 is a key splicing ...

BACTERIAL PROTEIN EXPRESSION E.coli expression cultures are typically 1.5-4.5 liters, depending on the expected yield. Cultures are typically grown in TB or LB at 37°C before down-tempering to 18 °C.

Previous in vitro purifications of the HsLARP6 La domain have either been single-step immobilized metal affinity chromatography (IMAC) purifications (16) or resulted in binding measurements ...

In their paper “Microfluidic Purification of Genomic DNA,” UF professors Jason Butler and Tony Ladd, along with their fourth-year Ph.D. candidate and research assistant, Jiayi Wang, detailed ...

The endeavor of protein expression and purification is a considerable investment of 2-3 weeks. In order to limit time waste, it is important to check expression and yield at each step.

Chhetri, G., Kalita, P. and Tripathi, T. (2015) An Efficient Protocol to Enhance Recombinant Protein Expression Using Ethanol in Escherichia coli. MethodsX, 8, 385-391.

Confirming the purification of biomolecules such as antibodies and proteins is desirable to characterize biological processes and ensure starting material quality.1.2 ...

Traditionally, downstream viral purification protocols often involved laborious steps such as density gradient ultracentrifugation - while sufficient for proof-of-principle experiments, such ...

The MEA 2 system can be used with PhyTip columns packed with a wide range of chromatography resins - such as Protein A, Protein G, IMAC, ProPlus, C18 and desalting gel filtration media. To ensure that ...