Nature2mon
A ubiquitin-based tagging system for controlled modulation of protein stability
Many biotechnology applications depend on the expression of exogenous proteins in a predictable and controllable manner. A key determinant of the intracellular concentration of a given protein is ...
Nature8mon
Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling
The thymidine kinase (TK) genes from herpes simplex virus (HSV) types 1 and 2 were recombined in vitro with a technique called DNA family shuffling. A high-throughput robotic screen identified ...
eLife5y
Temporal regulation of axonal repulsion by alternative splicing of a conserved microexon in mammalian Robo1 and Robo2
Proper connectivity of the nervous system requires temporal and spatial control of axon guidance signaling. As commissural axons navigate across the CNS midline, ROBO-mediated repulsion has ...
eLife5y
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
Recombinant DNA reagent pET-His6-ERK2 MEK1_R4F_coexpression vector Gift from Melanie Cobb Addgene plasmid Cat#39212 Recombinant DNA reagent S5A 13 repeat CTD Biomatik Cloned into pET28a derived vector ...