By using autologous tumor cells for vaccine generation, patients with all HLA haplotyps can be successfully immunized, when shared or unique tumor associated antigens are presented by the tumor.
PBMC were isolated and stimulated with IL-33, IL-12 and IL-23 and the cytokine profile response was examined by flow cytometry. Intracellular cytokine detection of IFNγ and IL-17 was performed by flow ...
Fluorescence-activated cell sorting (FACS) analysis was used to determine surface expression and intracellular cytokine content in purified CD14 monocytes. Membrane staining ... antibody specificity.