Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Flow cytometry workflows. ... refined intra- and extracellular staining protocols, and best-in-class antibodies. Still, human error, small differences in sample integrity, ...

Nature Protocols - Analysis of ... F. & Riccardi, C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271–279 ...

Protocols to prepare cells for single-cell suspension flow cytometry can be modified to detect antigens, ... centrifuged, and resuspended in a flow cytometry staining buffer.

Flow cytometry is a single cell technology that utilizes lasers and fluorescence (in most cases) to study the gene expression profile and function of cells. The power of flow cytometry is its ability ...

Flow Cytometry Protocols Editors: Hawley, Teresa S., Hawley, Robert G. (Eds.) ... What fixative do you need, do you need to study viability, staining first or after (intracellular or out, some ...

A new system for automated flow cytometry sample preparation, the Laminar Wash™ AUTO 1000, offers scientists a fully-automated flow cytometry staining platform designed to produce the most ...

Flow cytometry uses fluorescent probes to identify and characterize cells or particles in suspension (e.g. cells, nuclei or chromosomes) by virtue of size, granularity and fluorescence ...

Prepare 2x staining cocktail containing all of your labeled reagents at the right titer. The suggested titer for the MHC tetramer reagent is 1:200. Add 25ml of 2x staining cocktail. Mix by pipetting ...