Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

The Life Sciences Research Facility and the Nano Research Facility are home to the university’s Imaging and Flow Cytometry Core. This open access resource, developed and managed by DCU Core ...

Moreover, 2’F-araAC can induce G2/M cell cycle arrest and apoptosis in P388 and L1210 mouse leukemia cell lines as shown by flow cytometry method. Apoptosis was 54.53% and 43.35% for 2’F-araAC vs.

Many technical advancements have improved how flow cytometry is conducted, including improved flow cytometers for higher parameter analysis, refined intra- and extracellular staining protocols ...

However, for clinicians who are not in a research lab but want to collaborate or conduct research using flow cytometry, they often lack the resources, lab technicians, or knowledge to design a panel.

During protocol optimization, consecutive dilutions from 1,0E-04 to 1,0E-09, corresponding to ≥1,5E+09 to ≥1,5E+02, were evaluated by flow cytometry, and the dilution 1,0E-07 (corresponding to approx.

The small intestine pieces were incubated with EDTA solution (2mM) in HBSS, and the supernatants were recovered for the analysis of intraepithelial cells. These supernatants were washed once with PBS ...

Flow cytometry is a single cell technology that utilizes lasers and fluorescence (in most cases) to study the gene expression profile and function of cells. The power of flow cytometry is its ability ...

The protocol builds on existing work by Lu et al. and details the step-by-step procedure for the analysis of myeloid populations in mice tissue by flow cytometry.