Moreover, such endogenous biotin sometimes interferes with immunohistochemical evaluation by the avidin-biotin-peroxidase complex (ABC) method, even in the case of formalin-fixed and paraffin ...

Avidin-Biotin Complex (ABC) Method. Here, large complexes formed by avidin and biotin form linkages via reporter enzymes. These are then placed in an incubator along with biotinylated antibodies.

Biotin-binding proteins, including avidin and its bacterial counterpart streptavidin, are distinguished by their remarkably high affinity for biotin (vitamin B7). These proteins have been ...