The stain can be applied to live, detergent-permeabilized or ... With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning cytometry. The ability to ...

Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

View Full Profile. Learn about our Editorial Policies. “All of the methodology that existed before flow cytometry suddenly could be applied to the single cell,” said Thomas Jovin, a biophysicist at ...

Flow cytometry uses fluorescent probes to identify and characterize cells or particles in suspension (e.g. cells, nuclei or chromosomes) by virtue of size, granularity and fluorescence ...

By targeting surface markers on cancer cells, engineered synthetic receptors expressed on immune cells can be directed to ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

The decade saw the birth of the AIDS epidemic, and as attention focused on HIV, researchers needed a method to accurately and reproducibly characterize immune cells. Flow cytometry was suddenly thrust ...

The Cytometry and Cell Sorting Facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting. This site ...

The Flow Cytometry facility is directed by James Orth. For further information and training, please contact James Orth. Miltenyi Biotec MACSQuant VYB Analyzer: This is our newest instrument, equipped ...