This manuscript provides valuable information about the genesis of CPSF6 condensates due to HIV-1 infection. However, the evidence is incomplete as it is missing more functional assays. Furthermore, ...

The tissues were homogenized in extraction buffer (50 mM Tris–HCl [pH7.5], 50 mM KCl, and 10 mM MgCl2) supplemented with complete ... Invitrogen), mouse anti-vinculin (1:5000, # V9264, Sigma), rabbit ...

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Imaging buffer (20 mM Hepes pH 7.2, 137 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4, 6 mM D- glucose, 2 mM MgCl2, 2 mM CaCl2, 1% BSA) has been described before (2). Jurkat T cells were activated on 96-well ...