Dissociation is often misunderstood, even among mental health providers, largely because it’s not a widely taught or well-recognized subject in many clinical training programs. Yet, for many ...
Dissociation is more than a buzzword on social media. While the term is sometimes tossed around to describe feeling temporarily checked out, dissociation is a very real process that involves a ...
Imaging buffer (20 mM Hepes pH 7.2, 137 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4, 6 mM D- glucose, 2 mM MgCl2, 2 mM CaCl2, 1% BSA) has been described before (2). Jurkat T cells were activated on 96-well ...
To each well containing cells, 50 uL of CLB+hash solution (45 uL of Cold Lysis Buffer – 10mM Tris/HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL, 1% (v/v, Sigma-Aldrich, I8896), SuperaseIn RNase ...