Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan Division of Molecular Neuroimmunology, Medical Institute of Bioregulation ...
Introduction: As the golden approach of single-cell analysis, fluorescent flow cytometry can ... 5) in intracellular blocking, the same treatment was repeated as step 2; 6) in intracellular staining, ...
Design Flow cytometry, immunohistochemistry ... and cytokine receptors and genes associated with antigen presentation and macrophage polarisation. A similar phenotype (MerTK+MHCII high) with enhanced ...
Mass cytometry time-of-flight (CyTOF ... USA), and stained with an intracellular antibodies cocktail for 30 min on ice. Cells were washed and resuspended in deionised water, added into 20% EQ beads ...
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa223-8522, Japan Department of Biosciences and Informatics, Faculty of ...
Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China Department of Clinical ...
This study provides useful insights into the ways in which germinal center B cell metabolism, particularly lipid metabolism, affects cellular responses. The authors use sophisticated mouse models to ...
Such antibodies were detected by indirect immunofluorescence (IIF) on ethanol-fixed neutrophilic granulocytes revealing a pattern of perinuclear staining (P-ANCA ... At the end of the ‘80s, the ...
Adoptive immunotherapy using chimeric antigen ... flow cytometry assays that are suitable to characterize immune cell subpopulations in the peripheral blood during CAR-T cell treatment. The respective ...