Total protein for each spot is assessed by staining one in every 20 slides with Sypro Ruby Blot Stain (Molecular Probes, Eugene, OR) according to the manufacturer's directions. Slides are scanned on a ...

Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was widely ...

As a lab procedure, Southern blots can be used to analyze an organism's total DNA, also known as its ... breaking it into small fragments using a protein called a restriction enzyme.

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

We used a standard procedure for western blot analyses. We purchased antibodies against total p53 and Ser15-phosphorylated p53 from Transductional Signaling Cell, and antibodies against Bclx and ...