To exploit this wealth of data, we have cataloged the methods used in the refolding of some 150 proteins in a web-accessible database, REFOLD. The efficiency of the outlined methods has been noted ...
Both unfolded forms refold to produce the same native protein. It proved difficult to decide by refolding experiments whether or not proline isomerization explains the two unfolded forms of RNase ...
It was known that proteins unfold to cross mitochondrial membranes. Everyone assumed they then spontaneously refolded to the active form. But what if a “machine” helped the refolding? After all, ...
They will then discuss a novel method for solubilizing and refolding proteins using hydrostatic pressure to generate proteins whose structure matches that of the native protein, as confirmed by ...
doi: 10.1128/JVI.01528-19. PMID: 31723022; PMCID: PMC7000966. Lee ME, Dou X, Zhu Y, Phillips KJ. Refolding Proteins from Inclusion Bodies using Differential Scanning Fluorimetry Guided (DGR) Protein ...