The stain can be applied to live, detergent-permeabilized or ... With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning cytometry. The ability to ...

Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

View Full Profile. Learn about our Editorial Policies. “All of the methodology that existed before flow cytometry suddenly could be applied to the single cell,” said Thomas Jovin, a biophysicist at ...

By targeting surface markers on cancer cells, engineered synthetic receptors expressed on immune cells can be directed to ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

3b). Summary and future perspectives We have shown that cell entrapment into microdrops can be used for the quantification and isolation of cytokine-secreting cells by flow cytometry. This ...

The Cytometry and Cell Sorting Facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting. This site ...