Flow cytometry is a laser-based technique used to analyze ... The process involves preparing single-cell suspensions, ...
Multiplexing with these unstable fluorophores can impact conventional and spectral cytometry data ... Consistent staining using StarBright Dye conjugated antibodies within and between lots.
“If you compare multiple antibodies to different epitopes of a given target, you can very rapidly conclude what’s real staining and what’s not,” says Burmer. Research your reagent ...
However, choosing optimal antibody-fluorochrome combinations is a complex process. Here we provide some simple guidelines for the selection of reagent panels for multicolor flow cytometry.
We investigated the response of oligo-conjugated antibodies towards four variables: concentration, staining volume, cell number at staining, and tissue. We find that staining with recommended antibody ...
Keep the staining reactions at as low a volume as possible to conserve ... The CD8-mediated binding can usually be blocked by inclusion of select CD8 monoclonal antibodies that block the interaction.
The anti-FMC63 scFv monoclonal antibody’s performance was verified in house through flow cytometry (FCM), and ... His Tag Star Staining CD9-H8259 HEK293 Biotinylated Human CD19, Fc Tag, ultra ...
Spin-out ChromaTwist has won a prestigious Innovate UK Smart grant to co-fund a £0.5m project.  The funding from Innovate UK, the UK’s innovation agency, covers 70% of the cost of a project that will ...